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. 2016 Jul 3;23(5):415–425. doi: 10.1093/dnares/dsw024

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© The Author 2016. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Validation of differentially expressed milRNAs obtained in high-throughput sequencing by RT-qPCR/RT-PCR. (A) The expression of selected miRNAs (Rhi-milR-92, Rhi-milR-94, and Rhi-milR-151) was assayed in the first three stages and normalized to the 18S control gene. All analyses were performed with three biological replicates. Each bar represents the mean ± standard deviation (S.D.). (B) RT-PCR for three novel miRNAs (Rhi-milR-92, Rhi-milR-94, and Rhi-milR-151) providing experimental proof that these milRNAs exist in fungi.