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. 2016 Jul 3;23(5):415–425. doi: 10.1093/dnares/dsw024

Figure 4.

Figure 4

Validation of the predicted Rhi-milR-16 target. (A) Rhi-milR-16 secondary structure. (B) The Rhi-milR-16 targeting sequence in the 3'-UTR of AG1IA_02173 mRNA. (C) Rhi-milR-16 targets AG1IA_02173 through a targeting sequence located at its 3'-UTR. Dual-luciferase reporter assays were performed to test the interaction of Rhi-milR-16 and its targeting sequence in the 3'-UTR using constructs containing the predicted targeting sequence and a mutated targeting sequence cloned into the 3'-UTR of the reporter gene. The data represent three independent experiments with three measurements. * indicates P < 0.05.