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. 2016 Jul 8;23(5):495–505. doi: 10.1093/dnares/dsw034

Figure 5.

Figure 5.

A change in a conserved amino acid of Ehrlichia chaffeensis σ70 4.2 region further reduced the promoter activity in -35 motif mutants. The activities for the -35 motif mutants T1A, T2A and C4T of p28-Omp19 promoter were assessed with E. chaffeensis σ70 mutant (E597A) by measuring changes in the β-galactosidase expression in the E. coli strain CAG20177. The reduction of the enzyme activity was expressed relative to the wild-type promoter.