Figure 1. Dab2 expression in murine macrophages.

(a,b) Murine BM cells were obtained from mouse femur and tibia and were subject to ex vivo differentiation in the presence or absence of M-CSF. BMM were collected and analyzed by flow cytometry using the anti-F4/80 antibody (panel a, left). The lysates of BMM (panel a, right) and RAW264.7 (panel b) cells were analyzed by Western blotting using the anti-Dab2 (p96) antibody. The expression of β-actin was used for the control of equal protein loading. The full Western blot and the corresponding positions of the molecular weight protein markers are presented in Supplementary Fig. S2. (c) Dab2 localization in the RAW264.7 cells was analyzed by immunofluorescent staining followed by confocal microscopy analysis. Immunofluorescent staining was performed using the mouse anti-Dab2 (p96) primary antibody followed by incubation with the Alexa Fluor 488-conjugated anti-mouse IgG secondary antibody (green). Secondary antibody staining alone was represented as a negative control of fluorescent signal (upper panel). Nucleated cells were defined by positive fluorescent staining of DAPI (blue). Scale bar: 10 μm.