Figure 6. A Schematic diagram of TLR4 endocytic regulation by Dab2.

In the steady state, Dab2 sequesters clathrin from the TLR4/MD2 complex. TLR4 signals via two pathways in response to LPS. The surface TLR4 signal via MyD88 activates NF-κB and pro-inflammatory cytokine production. Clathrin is released from Dab2 and is associated with TLR4 upon LPS stimulation. TLR4/MD2 then undergoes endocytosis in a clathrin-dependent manner resulting in IRF3 phosphorylation and type I interferon production.