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. 2016 Oct 17;6:34879. doi: 10.1038/srep34879

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Copyright © 2016, The Author(s)

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Cells were sensitized with anti-DNP IgE and then stimulated with DNP-BSA at different time points. (A) Fluorescence microscopy image of cells pre-stained with dextran-FITC (green) and adipored (red). Scale bars represent 10 microns. Cells were sensitized with anti-DNP IgE, and then stimulated with DNP-BSA for 3 min. (D,E,I–K) 3D structure reconstructions from SXT. (B,C,F–H) Segment images of cells in SXT reconstructions. (D,E) A magnification from the rectangle in panel (B,C) in different orientation. Arrow indicates a cavity in the membrane protrusion region. Granules: red; mitochondria: green; granule fission: violet; cell membrane: dark yellow. Scale bars represent 1 micron. Nu: nuclear; G: granule.