Skip to main content
. 2016 Oct 17;6:34879. doi: 10.1038/srep34879

Figure 6. Granule-containing vesicles in mast cell degranulation by SXT and freeze substitution TEM.

Figure 6

Cells were sensitized with anti-DNP IgE and then stimulated with DNP-BSA for 30 min. (A) A segment image of a cell from SXT reconstructions. (B) A 3D image of Granule-containing vesicle from (A). (C–F) Magnification of I-IV in (A). (G) A TEM image of a section from freeze-substituted and Spurrs’ resin embedded cells. (H,I) Magnification of Granule-containing vesicles in part a and b from panel (G), respectively. Granule: red; mitochondria: green; GCV membrane: dark yellow; lipid bodies: blue. Scale bars represent 1 micron.