Figure 1. The transcriptome profile and the AggR regulon of the E. coli O104:H4 pAA plasmid.

Track 1: dRNA-seq profile of the pAA plasmid. The graphs represent the normalized number of pAA reads mapped per nucleotide in TEX+ (red) and TEX- (black) libraries (y-axis = abundance relative score, max. 100). Track 2. TSS (red) and PS (black) candidates mapped by dRNA-seq. Track 3. Computationally predicted AggR (red) binding sites. Track 4. Annotated ORFs in pAA (NC_018666.1). Virulence associated genes are colored in orange. Track 5. AggR regulon. The AggR regulon is based on Morin et al.¹⁶ (light orange) and our study (dark orange). The image was generated using Circos⁵².