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. 2016 Sep 1;30(17):1943–1955. doi: 10.1101/gad.283499.116

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© 2016 Liu et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — Loss of SMO in pancreatic fibroblasts induces ADM in vitro. (A) Images and quantitation of ductal-like structures in 3D ADM formation assay treated with the indicated CM. Insets show a higher magnification view of representative cellular structures. The graph represents quantitation of ductal-like structures. Bar, 40 μm. n = 3. Error bars represent means ± SD. (B) Matched H&E and coimmunofluorescence images of representative cellular structures from 3D ADM formation assay stained for CK19 (red) and β-amylase (green) after treatment with the indicated CM. (C) IHC and quantitation of phosphorylated EGFR (Tyr1068). The graph represents quantification of the H score for each genotype. Bar, 25 μm. n = 3. Error bars represent means ± SD. (D) Quantitation of ductal-like structures in 3D ADM formation assay treated with KCS CM and DMSO or erlotinib. Bar, 40 μm. n = 3. Error bars represent means ± SD. (**) P < 0.01; (***) P < 0.001.