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. 2016 Sep 1;30(17):1943–1955. doi: 10.1101/gad.283499.116

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© 2016 Liu et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Deletion of SMO in fibroblasts promotes ADM via TGF-α. (A) Quantitative real-time PCR analysis of known EGFR-activating ligands. n = 3. Error bars represent means ± SD. (B) Western blot of TGF-α in CM; SPARC was used for a loading control. (C) IHC and quantitation for TGF-α; the graph represents the quantification of the H score for each genotype. Bar, 25 μm. n = 3. Error bars represent means ± SD. (D) Images and quantitation of ductal-like structures in 3D ADM formation assay with pretreatment of KCS CM with TGF-α-neutralizing antibody. The insets show a higher magnification view of representative cellular structures. The graph represents the quantitation of ductal-like structures. Bar, 40 μm. n = 3. Error bars represent means ± SD. (*) P < 0.05; (**) P < 0.01; (***) P < 0.001.