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. 2016 Oct 17;6:35205. doi: 10.1038/srep35205

Figure 3. MiR-127 increased neuronal loss, promoted cell apoptosis and inhibited axonal regeneration after SCT.

Figure 3

Slices of rostral spinal cord derived from 28 dpo were subjected to immunohistochemistry for NeuN (a–d, red, white arrow), CGRP (e–h, red, white arrow), GAP-43 (i–l, red, white arrow), and counterstained with DAPI (blue) in sham group (a,e,i, n = 5), SCT group (b,f,j, n = 5), NS-miRNA (c,g,k, n = 5) and miR-127 group (d,h,l, n = 5). Three days post operation, TUNEL staining was used to analyze neuronal apoptosis (m–p, red, white arrow) in rostral of spinal cord in sham, SCT, NS-miRNA and miR-127 group. Sections were stained with DAPI (blue) to show all nuclei, and TUNEL (red, white arrow) to show apoptotic cells, in merged photomicrographs rose-red were defined as TUNEL positive. (q) The percentage of the NeuN+/DAPI was measured. (r,s) Mean density of CGRP (r) and GAP-43 (s), which presented as IOD/Area in each group were measured. (t) Quantitative histogram showed the percentage of TUNEL/DAPI in sham, SCT, NS-miRNA and miR-127 group. *P < 0.05, **P < 0.01. Scale bar: (a–d), 100 μm; (e–p), 50 μm.