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. 2016 Oct 17;6:35224. doi: 10.1038/srep35224

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Bf: Biceps femoris; Sol: Soleus. RPKM values are used to calculate the gene expression in RNA-seq and normalize the expression of one group to “1”. In real-time PCR, relative expression levels are calculated using ∆∆Ct value method and normalized by reference gene GAPDH, and similarly normalize the expression of one group to “1”. The data showed in Y axis represented the fold change. The unpaired Student’s t-test is used to evaluate the statistical significance of differences between the two groups, *P ≤ 0.05, **P ≤ 0.01. All data are presented as mean ± standard error (SE).