Figure 4. Plate assays performed on AMM agar (containing 20 mM L-glutamine as nitrogen source) for 48 h to test for sensitivity to various ROS inducing agents and heavy metals.

Concentrations of stress-inducing agents (hydrogen peroxide, H₂O₂; paraquat, PQ; menadione, MD; tert-butylhydroperoxide, TBHP) and metals (iron, FeSO₄; copper, CuSO₄; zinc, ZnSO₄; cobalt, CoCl₂) are given in mM; All strains are AfS77 derivatives and 10⁴ spores were spotted. In wild-type-background EGT deficiency (strain ∆egtA) impaired resistance against H₂O₂ and MD and in a ∆yap1-background (strain ∆egtA∆yap1; Yap1 is a transcriptional activator orchestrating oxidative stress defense²⁸) additionally against PQ and the metals copper, zinc and cobalt and iron. Complementation of EGT deficiency in the double mutant strain (egtA^c∆yap1) restored phenotype to that of a Yap1-lacking mutant.