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. 2016 Oct 17;6:35542. doi: 10.1038/srep35542

Figure 1. Effect of TGFβ1 on hMSC migration, proliferation and differentiation.

Figure 1

(a) Chemoatxis of hMSC in response to 0.01 pg/ml to 10 ng/ml TGFβ1 expressed as chemotactic index (number of cells per field of view normalised to the control). (b) Proliferation of hMSC treated with 0.1 pg/ml −20 ng/ml TGFβ1 expressed as DNA concentration. (c–e) Osteogenic differentiation of hMSCs after 14 days of treatment with 0.1 pg/ml −20 ng/ml TGFβ1. (c) qPCR analysis of Osteocalcin, Osteopontin, RunX2 and BMP2 expression. (d) Alizarin red staining, scale bar 200 μm. (e) Assessment of mineralisation by calcium concentration measurement. All results are expressed as mean ± SEM and analysed using 1 way Anova with Dunnett’s multiple comparison post-test, *p < 0.05, **p < 0.01, ***p < 0.001.