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. 2016 Oct 17;6:35456. doi: 10.1038/srep35456

Figure 5. SDS-PAGE immunoblot and stain-free gel images of different biochemical fractions of adult mouse cortex.

Figure 5

Samples of different enriched subfractions were resolved on SDS-PAGE, and then immunoblotted for specific synaptic markers. NMDA receptor 2b (Grin2b) and Dlg4 proteins are markers of postsynaptic density fraction; synaptophysin (Syp) is a marker of the presynaptic terminal. Hom: homogenate; P2: pellet 2; Syn: synaptosome; Sym, synaptic membrane; PSD: Triton X-100 insoluble postsynaptic density fraction. Normalization of the protein input was performed using the stain-free gel.