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. Author manuscript; available in PMC: 2017 Oct 6.
Published in final edited form as: Cell. 2016 Sep 29;167(2):369–381.e12. doi: 10.1016/j.cell.2016.09.017

Figure 1. Transient protein overexpression produces stable phenotypic states with prion-like patterns of inheritance.

Figure 1

A) Growth parameters (in order: lag, slope, yield, and area under curve) in stress conditions for cells harboring stable phenotypic states relative to naïve cells. B) Growth of cells harboring phenotypic states after curing by transient inhibition of Hsp104, Hsp70, and Hsp90. Error bars represent SE from three biological replicates. Blue– phenotypic state; dark grey– naïve; light grey– ‘cured’. Standard error ca. 15%. C) Phenotypic states (orange) are dominant in genetic crosses to isogenic naïve strains (gray) and exhibit non-Mendelian patterns of segregation (4:0 in tetrads). Segregation pattern of a spontaneous ZnSO4-resistant mutant that arose during the screen is shown as a point of comparison (2:2 Mendelian inheritance rather than 4:0). Growth rates shown in heat map. Standard error ca. 15%.