Figure 4. Proteins that induce phenotypic states do not form amyloid fibers.

A) Immunoblot of SDD-AGE run of lysates from diploid cells harboring stable phenotypic states and expressing integrated GFP-tagged copies of their inducing proteins at 18 h exposure. [PSI⁺] shows robust signal (30 min exposure). The first three lanes of the blot contain three independent isolates of the [PSP1⁺] prion, followed by [psp1^-], [SPC110⁺], [spc110^-], [HAA1⁺], [haa1^-], [HEH2⁺], [heh2^-], [BUD2⁺], [bud2^-], [RBS1⁺], [rbs1^-], [VTS1⁺], [vts1^-], [MPH1⁺], and [mph1^-]. B) Representative fluorescence micrographs from similarly constructed diploids.