(A) Expression of GFP and Dectin-1 in Cx3cr1gfp/+ mice during development (E8.5-10.5) in Kit+ cells (CD45low, Kit+), pMacs and macrophages. sp: somite pairs. Data are representative of n=9 independent experiments. Biological replicates have been aggregated per cell type, time point and tissue. (B) Flow cytometry analysis in Cx3cr1+/− and Cx3cr1−/− of pMacs and macrophages from yolk sac (YS), head, and caudal at E9.5 (upper panel) and liver, YS, head, and limbs at E10.5 (lower panel). Circles represent individual mice. Data are representative of n=6 independent experiments. P-values were calculated using Student’s t test. sp: somite pairs.