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. Author manuscript; available in PMC: 2016 Oct 17.
Published in final edited form as: Science. 2016 Aug 4;353(6304):aaf4238. doi: 10.1126/science.aaf4238

Fig. 5. Early specification of tissue-resident macrophages.

Fig. 5

(A) Flow cytometry analysis of Csf1rMeriCreMer; Rosa26LSL-YFP (OH-TAM at E8.5) liver, brain, lung and skin F4/80+ cells from postnatal mice (4 weeks old) showing expression of Il4ra, Il13ra1, Tnfr2, Ifngr, Dectin-1, CD64, Tim4, and CD206 (black dotted on whole population and green on YFP+ cells). Gray histograms show the fluorescence intensity of the FMO controls. (B, C) Scorecard analysis of all differentially upregulated genes in postnatal macrophages. The scorecards show the relative enrichment of each set of upregulated genes across each cell type (y-axis) and developmental time point (x-axis). See Methods for details of the score card. Numbers for each population indicate differentially up-regulated transcripts in postnatal (P2-P21) brain, liver, kidney, epidermis or lung macrophages when comparing one population vs. the others. See also Table S3. (D) Heatmap representing all differentially upregulated transcriptional regulators (2-fold change, adj. p-value<0.05, BH-correction) between postnatal macrophages from brain, liver, kidney, skin and lung macrophages, and their relative expression in tissue macrophages from E10.25 to P21.