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. 2016 Oct 17;6:35372. doi: 10.1038/srep35372

Figure 5. The effects of A779 and CFL1 siRNA pre-treatments on the autophagic activity in HAECs treated with angiotensin-(1-7) for 24 h.

Figure 5

(A) The expression levels of LC3b-I and LC3b-II in the control group and the groups pretreated with or without A779. (B) The immunoreactivity of Cofilin-1 and LC3b in HAECs treated with angiotensin-(1-7) for 24 h was stronger than in the control groups, whereas pre-treatment with A779 abolished this effect. (C) The expression levels of, LC3b-I and LC3b-II in the control group, the group that was treated with angiotensin-(1-7) for 24 h, the group that was pretreated with CFL siRNA transfection and then treated with angiotensin-(1-7) for 24 h and the group that was pretreated with the mock treatment and then with angiotensin-(1-7) for 24 h. (D) The immunoreactivity of Cofilin-1 and LC3b-II in HAECs treated with angiotensin-(1-7) for 24 h was stronger than in the control HAECs, whereas pre-treatment with CFL siRNA abolished the effects (Scale bar: 50 μm). All the above slides were double-stained for Cofilin-1 (green) and LC3b (red), and DAPI nucleic acid staining is shown in blue. (E) In the above 5 different groups, the gene expressions levels of LC3b as indicated by the qPCR were quantified. The data are expressed as the mean ± SEM (n = 5). *p < 0.05 indicates a significant difference relative to the individual control groups.