Figure 3. Vinculin associated focal point adhesions: Distribution of actin filaments and vinculin in human mesenchymal stem cells, showing cytoskeletal organization.

Actin filament, vinculin and nucleus are pseudocolor in green, red, and blue respectively. (A) 8^th day, MSCs seeded in microchannel without any shear stress. (B) 8^th day MSCs seeded in microchannel under 1 × 10⁻³ μbar. (C) 8^th day MSCs seeded in microchannel under 2 × 10⁻³ μbar. (D) 8^th day MSCs seeded in microchannel under 4 × 10⁻³ μbar. (E) Static Control (F) MSCs seeded in microfluidic device with cryopreservation treatment. Cryopreserved MSCs after 8 days of (G) 1 × 10⁻³ μbar, (H) 2 × 10⁻³ μbar, (I) 4 × 10⁻³ μbar shear stress inside microchannel. (J) Quantitative difference in focal points in MSCs at various shear stresses compare to static control. (NS: > 0.05, ** < 0.01, *** < 0.005).