FIG 4.

Competition assay between expressed CTV proteins and the GFP-labeled CTV virion for in vitro binding to dissected T. citricida foreguts and stylets. The fluorescence intensity of the competitive binding assay was assessed against the binding efficacy of the GFP-labeled CTV virion-only positive control. Horizontal thick lines indicate the medians, boxes show the interquartile ranges, including 25 to 75% of the values, and whiskers show the highest and the lowest values in each set. The circles represent outliers. Letters on the bars indicate significant differences (P < 0.05). To calculate the percentage of fluorescence density, the mean optical density of the positive control (mouthpart incubated with GFP-labeled virion without any competitor) was normalized to 100 (maximum fluorescence); then data points from all treatments were calculated relative to 100.