FIG 5.

The binding of CTV particles and foregut surface is similar to the lectin-sugar interaction. (A) Effect of the enzymatic treatment of dissected T. citricida foreguts and stylets on the in vitro binding of the GFP-labeled CTV virion. Five replicates of the each of four enzymatic treatments were tested at three different concentrations, and the fluorescence intensity was compared to that of the untreated GFP-labeled CTV virion-only positive control. (B) Competition between lectins and the GFP-labeled CTV virion for in vitro binding to dissected T. citricida foreguts and stylets. (C) Competition between sugars and the GFP-labeled CTV virion for in vitro binding to dissected T. citricida foreguts and stylets. Letters on the bars indicate significant differences (P < 0.05). To calculate the percentage of fluorescence density, the mean optical density of the positive control (mouthpart incubated with GFP-labeled virion without any competitor) was normalized to 100 (maximum fluorescence); then data points from all treatments were calculated relative to 100. ns, not significant; nU, nano-international unit.