TABLE 1.
Bacterial strains and plasmids used in the study
| Strain, plasmid, or oligonucleotide | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Dickeya dadantii strain 3937 | ||
| A350a | rafR ganB | 42 |
| A3092 | gmd::uidA Kanr | 29 |
| A4194 | rafR ganB phoP::Cmr | 13 |
| A5256 | rafR ganB arnB::uidA Kanr | 13 |
| A5394 | rafR ganB dltB::uidA Kanr | 3 |
| A5537 | rafR ganB arcA::Cmr | This work |
| A5632 | rafR ganB phoH::uidA Kanr | This work |
| A5749 | rafR ganB phoS::lacZ Kanr | This work |
| A5823 | rafR ganB dltD::uidA Kanr | This work |
| Plasmids | ||
| pGEM-T | PRC cloning vector | Promega |
| pBBR1-MCS5 | Gmr | 43 |
| Oligonucleotides | ||
| phoSfor | GTAGCGCACATTCATCTTGCC | |
| phoSrev | GGATAAGCGCCACACCAGAC | |
| arcAfor | CCCAAAGCGCCCGAGAACC | |
| arcArev | CTGGCGCATGATAAGCGCGC | |
| phoHfor | TGTCGTTTTTGCAGTGAAGTGTCC | |
| phoHrev | ATTAACACCGCCATCAACCAGATG | |
| alldltfor | GGAAGCTTTACCGTCTGGTGTGGCGC | |
| alldltrev | CCGAATTCGATGTTCTTCCTAACCGCG |
a
Strain A350 is a rafR and ganB mutant of strain 3937 devoid of β-galactosidase activity.