Table 1.
Descriptions of Assay 1 and Assay 2 used to measure plasma GPC3 levels
| Category | Assay 1 | Assay 2 |
|---|---|---|
| Manufacturer | USCN Life Science Inc | R&D Systems, Inc. |
| Capture antibody | Monoclonal mouse | Monoclonal mouse |
| Detection antibody | Polyclonal rabbit | Polyclonal sheep |
| Plate | Pre-coated with capture antibody | Manual capture antibody coating |
| Incubation temperature | 37°C | Room temperature |
| Biotin binding protein | Avidin | Streptavidin |
| Wash | Aspirate but no wash between adding sample and detecting antibody | Aspirate and wash between adding sample and detecting antibody |
| Detection range | 0.156 ng/mL – 10 ng/mL | 0.312 ng/mL – 20 ng/mL |
| Sample dilution factor | 1:1 | 1:100 |
ELISA, enzyme-linked immunosorbent assay.