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. Author manuscript; available in PMC: 2016 Oct 17.
Published in final edited form as: Exp Eye Res. 2006 Dec 4;84(2):349–360. doi: 10.1016/j.exer.2006.10.009

Fig. 1.

Fig. 1

FIZ1 protein is present in several non-ocular tissues that express Fiz1 mRNA. Expression of FIZ1 protein in mouse tissues was examined by Disc-PAGE and immunoblotting with affinity-purified antibody to the FIZ1 protein. A. FIZ1 protein was detected in all tissues examined. (25 µg total protein per lane) Equivalent loadings were confirmed by direct staining of protein on the blot itself as described in methods. B. Dot blots of protein extracts from CV-1 cells transiently expressing the bovine FIZ1 protein used for production of the FIZ1 antibody. Lysates of cells transfected with 0 µg, 0.6 µg and 1.2 µg of the pDest26-bFiz mammalian expression plasmid were dotted onto nitrocellulose, and then incubated with or without (control) affinity-purified antibody to FIZ1.