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. Author manuscript; available in PMC: 2016 Oct 17.
Published in final edited form as: Exp Eye Res. 2006 Dec 4;84(2):349–360. doi: 10.1016/j.exer.2006.10.009

Fig. 5.

Fig. 5

FIZ1 activation synergy with NRL and CRX at the Rho and PDE6B promoters.

A. Fold activation of the Rho proximal promoter in co-transfected CV-1 cells. FIZ1 synergy required the presence of both NRL and CRX. All transfection mixes contained the Rho-promoter luciferase-reporter construct, pBR-130luc, and the normalizer Renilla-luciferase construct pRL-CMV. Triplicate sample wells were co-transfected with expression vectors for NRL, CRX, and FIZ1 in the combinations as indicated (expression vector +, corresponding empty vector −). Firefly luciferase activity was normalized to Renilla-luciferase activity for each sample. Fold Activation is shown relative to reporter DNA in the presense of all empty vectors. Bars indicate SD (n = 3).

Fold activation of the PDE6B minimal-promoter in co-transfected CV-1 cells. FIZ1 synergy required the presence of NRL, and was inhibited by CRX. All transfection mixes contained the PDE6B-promoter firefly-luciferase reporter, pGL2-PDE6B-luc, and the normalizer Renilla-luciferase construct pRL-CMV. Triplicate sample wells were co-transfected with expression DNAs for NRL, CRX, and FIZ1 in the combinations as indicated (expression vector +, corresponding empty vector −). Firefly luciferase activity was normalized to Renilla-luciferase activity for each sample. Fold Activation is shown relative to reporter DNA in the presence of all empty vectors. Bars indicate SD (n = 3).