Table 2.
β-Actin | TGACAGGATGCAGAAGGAGA | CGCTCAGGAGGAGCAATG | 75bp |
Fiz1 | TGTAGCGAATGTGGCAAGAGTTTC | AGGGCTTTTCACCAGTGTGGAC | 247bp |
Thermo Cycling |
95°C/10 min., 40 × (95°C/30sec., 62°C/45sec., 72°C/30sec.), melting curve (90°C – 50°C) |
β-Actin | TGACAGGATGCAGAAGGAGA | CGCTCAGGAGGAGCAATG | 75bp |
Fiz1 | TGTAGCGAATGTGGCAAGAGTTTC | AGGGCTTTTCACCAGTGTGGAC | 247bp |
Thermo Cycling |
95°C/10 min., 40 × (95°C/30sec., 62°C/45sec., 72°C/30sec.), melting curve (90°C – 50°C) |