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. Author manuscript; available in PMC: 2016 Oct 17.
Published in final edited form as: Exp Eye Res. 2006 Dec 4;84(2):349–360. doi: 10.1016/j.exer.2006.10.009

Table 2.

Primers for quantitative PCR analysis of mouse retinal gene expression using Roche SYBR green and β-actin comparitor.

β-Actin TGACAGGATGCAGAAGGAGA CGCTCAGGAGGAGCAATG 75bp
Fiz1 TGTAGCGAATGTGGCAAGAGTTTC AGGGCTTTTCACCAGTGTGGAC 247bp
Thermo
Cycling
95°C/10 min.,
40 × (95°C/30sec., 62°C/45sec., 72°C/30sec.),
melting curve (90°C – 50°C)