Figure 2.

Roscovitine enhances axonal regrowth of pre-injured rat primary dorsal root ganglia (DRG) sensory neurons. (A) Neurons isolated from rat L4–L6 DRGs 3 days after sciatic nerve transection were cultured on laminin and poly-L-lysine coated cover slips in primary neuron media, containing N-2 supplement, BSA and 0.2 ng/ml NGF, in the absence or presence of roscovitine (0.2, 2.0 or 10 μM). After 48 h, cells were fixed in 4% paraformaldehyde and immunostained with β-III tubulin antibody. Scale bar = 50 μM. (B) Axon lengths of DRG neurons exposed to different concentrations of roscovitine. Values are means ± SD of three independent experiments. Statistical significance using student’s t-test was set at p < 0.05*.