Fig. 8.

Quantitative RT-PCR analyses of riboflavin synthesis genes in roots of wild-type Edisto (A, C, E, G, and I) and fefe mutant (B, D, F, H, and J) after 3 days of treatment. The results were normalized to ubiquitin as an internal reference. The transcript abundance of each gene (means±SE, n=3) of both genotypes was normalized to Edisto root samples of the control treatment (10 μM Fe and 0mM bicarbonate). * and ** indicate statistical significance at P<0.05 and P<0.01, respectively, comparing bicarbonate treatments within the same Fe supply; Ŧ and ŦŦ indicate statistical significance at P<0.05 and P<0.01, respectively, comparing different Fe supply within the same bicarbonate treatment.