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Some genes show abnormal pre-mRNA splicing in both gfa1-1/+ and rid1-2/+ mutants. The abnormal retention of first introns in pre-mRNA of RNA splicing-related and female gametophyte development-related genes in rid1-2/+ and gfa1-1/+ mutants were detected by qRT-PCR analysis. The expression of TUBULIN2 was used as the internal control. All experiments were repeated three times.
