Fig. 2.

Reactive oxygen species (ROS)/malondialdehyde (MDA) detection and characterization of root hairs in root tips of the B-efficient genotype ‘QY10’ and the B-inefficient genotype ‘W10’ seedlings under a hydroponic culture system. (A–D) Dihydroethidium (DHE) staining in the roots of the seedlings grown under high (25 μM) and low B (0.25 μM) conditions for 10 d. The images show representative individuals of two independent experiments, with at least five seedlings examined for each experiment. The fluorescence intensity was scanned using the ImageJ plot profile (https://imagej.nih.gov/ij/). The x-axis is distance (cm) from the root caps, and the y-axis is relative pixel intensity. (E, F) MDA levels in the shoots (E) and roots (F) of ‘QY10’ and ‘W10’. Data presented are the means (n=3), and error bars denote the standard deviations. (G, H) Comparative analyses of the density and length of root hairs (G) and the length of non-root-hair zones (NRHZs) (G, H) between ‘QY10’ and ‘W10’. The root images in (G) correspond to the data presented in (H), which are the means (n=10), and error bars denote the standard deviations. The significance level was set at a P-value < 0.05. (This figure is available in colour at JXB online.)