Fig. 5.

Ghd2 activated the expression of SAGs in protoplasts. Effector (Ghd2) and reporter (promoter) constructs were co-transformed into rice protoplasts using a dual firefly-Renilla luciferase system. (A) Trans-activation activity of Ghd2 in rice protoplasts. 35S::Gal4BD:Ghd2 was co-transformed with Gal4::LUC and strongly induced LUC expression. (B–H) 35S::Ghd2 induced the expression of LUC driven by the promoters of SAGs under normal and ABA treatment conditions. The white bar shows the LUC activity of the empty effector vector and the grey bar the LUC activity of 35S::Ghd2 co-transformed with the reporter constructs.