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. 2016 Sep 16;67(19):5785–5798. doi: 10.1093/jxb/erw344

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Identification of Ghd2-interacting proteins. (A) 14-3-3 proteins (GF14b, GF14c, and GF14e), OsPURα, and OsARID3 interact with C3 or N3 deletions of Ghd2 in Y2H assays. Colonies grown on Synthetic complete-Leu-Trp medium (SC/-LT) were tested by both X-gal assay and selection on Synthetic complete-Leu-Trp-His medium (SC/-LTH) containing 5 and 20mM 3-AT for N3 and C3, respectively. (B) 14-3-3 proteins (GF14b and GF14c) and OsARID3 were confirmed to interact with Ghd2 by BiFC. YFP, yellow fluorescent protein. Bar = 20 μm. (C) Confirmation of the interaction between Ghd2 and OsARID3 by an in vivo pull-down assay. (D) Expression of ICL and MS in the OsARID3-OE and WT plants. (E) Expression analysis of OsPURα, OsARID3, and 14-3-3 proteins under drought stress conditions. This figure is available in colour at JXB online.