Fig. 1.

Induced expression patterns of the nicotine synthetic gene PMT1 and PR protein genes in the roots of wild-type tobacco Burley 21. (A) Expression pattern assay by semi-quantitative RT-PCR. The representative results of three independent replicates are shown. The sizes of the amplified products are indicated on the right. (B) Transcript levels of PMT1 and PR protein genes based on qRT-PCR analysis. Ctrl indicates untreated control. JA and ACC indicate treatment with JA and ACC, respectively. The transcription level of each gene in the Ctrl is set as ‘1’. Actin was used as an internal control for both semi-quantitative RT-PCR and qRT-PCR.