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. 2016 Sep 23;67(19):5799–5809. doi: 10.1093/jxb/erw345

Fig. 2.

Fig. 2.

Transcription patterns of PMT1 and PR protein genes in the wild type and in low-nicotine mutants of Burley 21. (A) Expression patterns of PMT1 and PR protein genes in roots by semi-quantitative RT-PCR assay. (B) Expression levels of PMT1 and PR protein genes determined by qRT-PCR. The transcription level of each gene in the Ctrl is set as ‘1’. (C) Transcripts of PR3b gene in leaves. (A, C) The representative results of three independent replicates and the sizes of the amplified products are indicated on the right. WT indicates wild type Burley 21. nic1, and nic2 indicate low-nicotine mutant alleles of NIC1 and NIC2, and nic1nic2 indicates the double mutant. The asterisk indicates primer dimers in the amplification products of the PR3b gene. Actin was used as an internal control.