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. 2016 Sep 23;67(19):5799–5809. doi: 10.1093/jxb/erw345

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Genetic linkage between alternative splicing of PR3b and the NIC1 locus in the Burley 21 background. (A) Alternative splicing of PR3b in individual F₂ plants of a cross between nic1 and wild-type Burley 21. (B) Transcript levels of PMT1 in the roots of individual F₂ plants. Transcript level of PMT1 in the roots of wild-type Burley 21 was set as ’1’. Actin was used as an internal control. (C) Leaf nicotine content of individual F₂ plants. The values shown are the means of three technical replicates. Error bar, mean ±SD.