Fig. 6.

Phytohormone-induced splicing patterns of PR3b. (A) Splicing patterns of PR3b in the wild type (WT) and in low-nicotine mutants (nic1, nic2, and nic1nic2). The representative results of three independent replicates are shown. The asterisk indicates the primer dimers in the PCR amplification products. (B) Quantification of specific transcript of native PR3b. (C) Quantification of specific transcript of spliced PR3b. Ctrl indicates the untreated control; JA, ACC, and JA+ACC (the combination of JA and ACC) indicate different phytohormone treatments. The PR3b transcript level in the WT of the Ctrl treatment is set as ‘1’ (B, C). Actin was used as an internal control.