Clinical biochemical parameters and histological analyses. (A) Serum creatinine, urea, uric acid, calcium and phosphorus of basic serum biochemical parameter and WBC, RBC, HGB and HCT of basic blood routine parameters comparisons between control and AAN groups. (B) Kidney tissues were stained using PAS method in control group and AAN group at weeks 4, 8 and 12. (C) Kidney tissues were stained using Masson staining in control group and AAN group at weeks 4, 8 and 12. All values presented as mean±SD. All statistical significances were calculated by two-tailed Student’s t-test with 95% confidence interval. *P<0.05, **P<0.01 compared to control group.