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. Author manuscript; available in PMC: 2017 Oct 5.
Published in final edited form as: Neuron. 2016 Oct 5;92(1):31–44. doi: 10.1016/j.neuron.2016.09.033

Figure 6. Interneuron clones in Harwell et al. exhibit a clustering feature in spatial distribution; See also Figure S7 and Table S2.

Figure 6

(A) Dendrograms of the left and right hemispheres of the single barcoded experimental dataset according to the Euclidean distances of multi-cell clones in the same brain structure. Numbers and colors indicate the lineal relationship between two or more cells based on the recovered barcodes. Colored lines below the numbers indicate spatially isolated clonal clusters. Colored dots mark a sibling neuron located away from the corresponding clonal cluster. Broken black lines indicate local clonal clusters occupying the same or nearby space. (B) Quantification of the percentage of local clusters (i.e. the lowest hierarchical branch in the dendrogram) that are clonally related in the experimental dataset (red bars) compared with the percentage estimated from random permutations of the clonal identity in the same dendrograms repeated for 100 rounds (black bars). Data are shown as the total percentage in the whole dataset as well as mean ± S.D. (n= 2 hemispheres). (C) Quantification of the percentage of clones that form local clusters in the experimental dataset (red bars) compared with the percentage estimated from random permutations of the clonal identity in the same dendrograms for 100 rounds (black bars). Data are shown as the total percentage in the whole dataset as well as mean ± S.D. (n=2 hemispheres). (D) Histograms showing the frequency of local clonal clustering estimated from random permutations of clones. Red arrows indicate the number of local clonal clusters observed in the experimental dataset. (E) Quantification of the average intra-clonal and inter-clonal Euclidean distances for individual clones in each experimental dataset. The “inter-clonal” and “intra-clonal” distances for single cells represent the average distance between individual clones and all single barcoded cells as a “clone”. Each black dot represents the average distance for an individual clone. Red lines represent mean ± S.D. (*, p<0.05; ***, p<0.001; n.s., not significant). (F) Quantification of the average inter-clonal and intra-clonal distances for all clones and single cells. Data are presented as mean ± S.D. (n=2 hemispheres; *, p<0.05; **, p<0.01; ***, p<0.001; n.s., not significant).