Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 15;30(18):2062–2075. doi: 10.1101/gad.283317.116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Li et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 3. — HSF-1 directly regulates genes essential for C. elegans larval development. (A) Relative expression of HSF-1-associated genes in wild-type (N2), hsf-1(ok600), and hsf-1(ok600); rmSi1[hsf-1::gfp] L2 animals (30 h after egg lay) at 20°C. Gene expression levels were determined by RNA-seq and normalized to that in hsf-1(ok600). (B,C) Venn diagrams showing the overlap of genes down-regulated (B) or up-regulated (C) in hsf-1(ok600) compared with N2 (FDR 0.05) and genes associated with HSF-1. P = Fisher's exact test. (D) The gene network directly regulated by HSF-1 in C. elegans larval development. HSF-1 directly activated genes corresponding to those genes directly associated with HSF-1 and down-regulated in hsf-1(ok600) (indicated in blue ovals); HSF-1 directly repressed genes corresponding to genes associated with HSF-1 and up-regulated in hsf-1(ok600) (indicated in yellow ovals). Solid lines represent physical protein interactions; dashed lines represent genetic interactions or coexpression. The 22 genes labeled in red are known to be required for larval development in genetic analyses. The eight genes induced upon heat shock are indicated with a purple outline around either blue or yellow ovals.