Figure 7.

Genome-wide analysis identifies endogenous GMD substrates. (A) Venn diagrams representing the number of differentially expressed transcripts. HeLa cells depleted of GR, YBX1, or HRSP12 were either treated or not treated with Dex for 1 h. Total-cell RNAs were purified and subjected to mRNA-seq. Transcripts obtained from two independent biological replicates were analyzed and selected using the following criteria: at least twofold down-regulation upon Dex treatment and at least 1.5-fold up-regulation upon down-regulation of GR, YBX1, or HRSP12. (B) Gene ontology analyses using the WEGO software of 139 transcripts that were down-regulated by at least twofold upon Dex treatment and commonly up-regulated by at least 1.5-fold upon down-regulation of GR, YBX1, or HRSP12. (C,D) The effect of YBX1 and HRSP12 on chemotaxis of THP-1 cells. (C) The cells adherent to the membrane were stained. Bars, 25 µm. (D) The chemotaxis index was calculated by counting the stained cells. The chemotaxis index values obtained in the absence of Dex were arbitrarily set to 100%. n = 2. (*) P < 0.05. (E) Model illustrating the sequential recruitment of GMD factors. The details are described in the Discussion.