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. 2016 Nov;22(11):1689–1698. doi: 10.1261/rna.056804.116

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© 2016 Coey et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 1. — (A) The primer tRNA^Lys₃ anneals to the vRNA at the PBS site to form an 18-bp helix from which reverse transcription initiates. This site is found within the highly structured 5′ UTR. The proximal locations of the conserved vRNA PBS and PAS sequences are highlighted in red and blue, respectively. (B) The sequences of the 77-nt-vRNA construct and tRNA^Lys₃ primer contain the complementary PBS/anti-PBS and PAS/anti-PAS sequences highlighted in red and blue, respectively.