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. 2016 Nov;22(11):1689–1698. doi: 10.1261/rna.056804.116

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© 2016 Coey et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 3. — (A) The 5′ side of the PAS oligo was constructed from the vRNA PAS sequence and the 3′ side from the tRNA anti-PAS sequence. The stem is capped by a UUCG tetraloop on one end and a GC base pair on the other. (B) Assigning the homonuclear NOESY spectrum of the PAS oligo shows that it folds into a single stem–loop structure. (C) Overlaying the PAS oligo NOESY spectrum (red) onto the 77-nt-vRNA/tRNA NOESY spectrum (black) shows that the 77-nt-vRNA/tRNA complex contains a PAS/anti-PAS secondary structural interaction. (D) NOESY data show that the vRNA PAS sequence displaces the tRNA 5′ end to base pair with the tRNA anti-PAS sequence.