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. 2016 Nov;22(11):1710–1718. doi: 10.1261/rna.057646.116

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© 2016 Binzel et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — Overview of the Phi29 pRNA and the three-way junction (3WJ). (A) (i) Secondary structure of the phi29 pRNA monomer with the pRNA-3WJ outlined by the box, which connects the helical domain to the interlocking procapsid binding domains. (ii) Secondary structure and sequence of the pRNA-3WJ and (iii) the crystal structure of the pRNA-3WJ. (B) Assembly gel of the pRNA-3WJ from the three short RNA oligo strands on 12% native PAGE. The 3WJ (lane 7) shows efficiency of folding and particle homogeneity before purification.