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RNase III-mediated cleavage of dinQ and agrB RNA. (A,B) 4.4 pmol of 3′ end labeled +1 dinQ or +44 dinQ RNA, alone or mixed with a twofold excess of antisense agrB RNA in a final volume of 17.5 µL, was treated with 0.001 U of RNase III for different times as indicated. (C,D) 6.6 pmol of 3′ end labeled agrB RNA, alone or mixed with an equimolar amount of +1 dinQ or +44 dinQ RNA in a final volume of 26.3 µL, was treated with 0.005 U of RNase III for different times as indicated.
