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. 2016 Jan 23;14(7):1633–1645. doi: 10.1111/pbi.12526

Figure 1.

Figure 1

The phenotype of MdbHLH104 transgenic apple plantlets under Fe‐sufficient and deficient conditions. (a) Expression level of the MdbHLH104 gene in the transgenic apple lines and the wild‐type (empty vector WT control). (b) The level of the MdbHLH104‐GFP fusion protein in 35S::MdbHLH104‐GFP transgenic apple lines, as determined by immunoblot analysis using an anti‐GFP antibody. The anti‐actin antibody was used as loading control. (c) The appearance, total chlorophyll contents and iron contents of 35S::MdbHLH104‐GFP transgenic apple lines and the WT control grown for 1 month on Fe‐sufficient (+Fe) or Fe‐deficient (−Fe + Frz) media. The data represent the means ± SD of three independent experiments. DW: dry weight. (d) The rhizosphere acidification and PM H+‐ATPase activity of wild‐type and 35S::MdbHLH104‐GFP transgenic apple lines grown for 7 days on Fe‐sufficient (+Fe) or Fe‐deficient (−Fe + Frz) media. The yellow colour around the roots stained with bromocresol purple indicates rhizosphere acidification, and the plasma membrane vesicles were isolated for PM H+‐ATPase activity analysis. The data represent the means ± SD of three independent experiments.