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. 2016 Jan 23;14(7):1633–1645. doi: 10.1111/pbi.12526

Figure 3.

Figure 3

MdAHA8 positively regulates PM H+‐ATPase activity. (a) The relative expression of MdAHA8 in wild‐type, pIR‐MdAHA8 and pIR‐MdAHA8‐Anti transgenic apple calli. The data represent the means ± SD of three independent experiments. (b) Acidification analysis of MdAHA8 transgenic apple calli treated on medium containing the pH indicator dye bromocresol purple. Acidification is indicated by yellow colour around the apple calli. The same is true below unless otherwise indicated. (c) PM H+‐ATPase activity of wild‐type, pIR‐MdAHA8 and pIR‐MdAHA8‐Anti transgenic apple calli grown for 7 days on Fe‐sufficient (+Fe) or Fe‐deficient (−Fe + Frz) media.