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. 2016 Jan 23;14(7):1633–1645. doi: 10.1111/pbi.12526

Figure 4.

Figure 4

MdAHA8 is required for MdbHLH104‐mediated acidification and iron contents of responding iron deficient. (a) Acidification of wild‐type, 35S::MdbHLH104‐GFP and 35S::MdbHLH104‐GFP/pIR ‐MdAHA8‐Anti transgenic apple calli. (b) PM H+‐ATPase activity in vesicles isolated from wild‐type, 35S::MdbHLH104‐GFP and 35S::MdbHLH104‐GFP/pIR ‐MdAHA8‐Anti transgenic apple calli treated with (+Fe) or without (−Fe + Frz) iron for 7 days. (c) Visualization of ferrous of Fe‐sufficient and Fe‐deficient conditions in wild‐type, 35S::MdbHLH104‐GFP and 35S::MdbHLH104‐GFP/pIR ‐MdAHA8‐Anti transgenic apple calli by FerroZine. The resulting Fe(II) is trapped by FerroZine to produce a red product. Fe content of wild‐type, 35S::MdbHLH104‐GFP and 35S::MdbHLH104‐GFP/pIR ‐MdAHA8‐Anti grown on Fe‐sufficient (+Fe) or Fe‐deficient (−Fe + Frz) media for 7 days. The data represent the means ± SD of three independent experiments. DW, dry weight.